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Please Select One of The Above
Topics: |
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Book a Demo
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Take a hands-on tour of the
outstanding features offered by our bioinformatics
products and/or LIMS software. |
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More  |
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HIT™ Non-Heat Shock Competent Cells
(1-10 minute Transformation)
The original transformation protocol dates back to 1970
(Mandel and Higa, J. Mol Biol 1970 , 53; 159). Before, just
preparing the cells took two days. With a new protocol two hours
long, containing multiple steps, it was a revolution in
molecular biology. Does this sound familiar? That's because
it's still the most popular protocol in use today.
| More than 30 years later we think it's time for another
revolution.
HIT™ Competent Cells are the fastest transforming
cells worldwide. Due to RBC's unique production process cells
can be transformed to high efficiency in 1-10 minutes using
a simple 1 step patented protocol. We provide a wide range
of popular strains for all your cloning and expression
needs. Transform your work with HIT™ Competent Cells. |
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RBC
HIT™ Advantage
Compared to traditional
Commercial Competent Cells
* Revolutionary 1 minute
Transformation
* First biotech company worldwide to ensure -70°C
Monitoring of competent cell shipments
* 1 step/1 tube process
* Guaranteed efficiency up to 109 cfu/ug
plasmid
* Each batch efficiency checked during production
* Economic pricing for regular and high efficiency
cells
* Ideal for bench users to automated cloning
projects
* Worldwide patent protection |
Compared to traditional DIY
Competent Cells
* Transform in 1 minute
* Save 2-3 days preparation time
* Guarantee efficiency
* Guarantee correct strain
* Select from a wide range of strains
* Economic HIT™ VALUE 108 kits
available |
RBC HIT™ Protocol Comparison Table |
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| Competent Cell Types |
HIT™
Competent Cells |
Traditional Chemically
Competent Cells |
Electro- Competent Cells |
| Time |
1 minutes |
1.5~2.5 hours |
1.5~2.5 hours |
| Protocol steps |
-One
-No tube change/SOC |
-Multiple
-Tube change/ SOC |
Multiple
-Tube change/SOC |
| Authentic representation |
High |
Requires
recovery step
|
Requires
recovery step
|
| Transformation Efficiency |
107~109/µg
pUC19 |
107~109/µg
pUC19 |
1011/µg
pUC19 |
| Stability of refrozen cells |
High |
Low |
Low |
| Stability of vortex |
Stable |
Unknown |
Unknown |
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HIT™ Competent Cells Protocol |
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1
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Prepare ice bucket, 37°C
plating beads and selective plates. Thaw
competent cell vial with room-temp. tap water
or water bath for 10~20 seconds until 1/3
thawed.
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2
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Add DNA whose volume is less
than 10% of volume of cells . Vortex 1 second.
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3
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Place on ice for 1-10 minutes
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4
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Transfer onto 37°C dry
selection plate media, spread using RBC
plating beads.
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5
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Immediately incubate plate at
37°C (16-18 hours for HIT-DH5a, 8-24 hours
for HIT-JM109). Observe growth of transformed
colonies.
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| Genotypes |
Applications |
HIT™-DH5a |
HIT™-JM109 |
HIT™-Blue |
HIT™- 21 |
| F- ( 80d lacZ M15)
(lacZYA-argF)U169 hsdR17(r - m +) recA1 endA1
relA1 deoR |
F'traD36proA+proB+ lacIq
(lacZ)M15 l (lac-proAB) hsdR17 recA1 endA1
relA1 |
hsdR17(rk- mk+), recA1,
endA1,
gyrA96,thi-1,supE44,relA1
,ac[F'proAB acIqZDM15Tn10(Tet )] |
E.coli B, F-, dcm, ompT,
hsdS(rB-mB-), gal (DE3 |
| endA |
Prevents plasmid degradation during
extraction |
Yes |
Yes |
Yes |
No |
| recA |
Prevents DNA recombination |
Yes |
Yes |
Yes |
No |
| hsd |
Enhances transformation efficiency of
selected PCR DNA strands and cDNA libraries |
Yes |
Yes |
Yes |
Yes |
| deoR |
Enhances transformation
efficiency of high MW
plasmids and cosmids |
Yes |
No |
No |
No |
| LacZ M15 |
Blue-White screening |
Yes |
Yes |
Yes |
No |
| Lon |
on Protease Deficient,
Improves Protein Yield |
No |
No |
No |
Yes |
| ompT |
OmpT Protease Deficient,
Improves Protein Yield |
No |
No |
No |
Yes |
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RBC HIT™ Application Table (also see
Genotype Table) |
|
| Cloning Applications |
HIT™-DH5a |
HIT™-JM109 |
HIT™-Blue |
HIT™-21 |
| Large Plasmids > 6Kb* |
Ideal |
Yes |
Yes |
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| Subcloning |
Ideal |
Ideal |
Yes |
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| cDNA library |
Yes |
|
Yes |
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| Fast Growth |
|
Ideal |
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| Single Stranded DNA |
|
Ideal |
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| Gene Bank |
Yes |
Ideal |
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| Mutagenesis |
Yes |
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| Protein Expression |
No |
No |
No |
Ideal |
| Blue/White Screen |
Yes |
Yes |
Ideal |
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To order or for further
information email
or call 800 717 0438 toll free in Canada and the USA. To contact your local Application Scientist, click here. |
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