Cloning

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Cloning utilizing vectors are the most popular method to obtain the scientist's gene of interest. While gene synthesis is gaining in popularity synthesized genes often require expression, further amplification and manipulation.

T&A Cloning System

This cloning system is ideal for cloning amplified products generated using a thermostable DNA polymerase, such as RealTaq Polymerase, which adds a single terminal 3'-dA nucleotide overhang. The kit contains all the components needed such as T4 ligase, vector and buffers. Optimal ligation time is 30 minutes, ligation can proceed within 5-15 minutes or up to 1 hour according to user. It is recommended to check ligation on an agarose gel. The vector confers ampicillin resistance and blue/white screening capability. RBC also provide the T&A vector as a stand alone product.

Fast Ligation Kit

The Fast Ligation Kit is used for both blunt and sticky end cloning. Ligation using the high quality T4 DNA ligase and optimized buffers can proceed within 5 minutes although 20-30 minutes may be optimal for certain sequences. Ligation product may be used instantly for transformation or purified to achieve increased efficiency.

Benefits of Our Cloning Kits

Economics: Unbeatable value for regular cloning.
Convenient: Accept amplified, blunt and sticky end DNA.
Stable: T&A Vector backbone is stable and regularly sequenced.
Rapid: Ligation can be achieved within 5 minutes for RBC Rapid Ligation.
Versatile: Accepts a wide size range of inserts.

Applications

	T&A Cloning Kit	T&A Vector	RBC Rapid Ligation Kit
PCR amplified DNA (Taq)	Yes	Yes	No
Terminal dA Tailed (Taq)	Yes	Yes	No
PCR amplified DNA (Pfu)	No	No	Yes
Blunt end-dephosphorylated	No	No	Yes
Sticky end	No	No	Yes
Linker Ligation	No	No	Yes

Note: The use of the PCR process is covered by various patents obtained by Hoffman-La Roche. PCR is a trademark of Hoffman-La Roche.

To order or for further information email or call 800 717 0438 toll free in Canada and the USA. To contact your local Application Scientist, click here.

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