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Characteristics
High purity nucleotides for use in PCR, RT-PCR, RT assay, DNA labeling reactions and sequencing / cycle sequencing analysis.
dNTP-01 - Is a premixed solution containing the sodium salts of dATP, dCTP, dGTP, and dTTP, > 99% HPLC.
- Size : 4 x 250 ul
- Concentration: 100 mM
- Storage: -20°C
Chemical Data
dATP
dCTP
dGTP
dTTP |
Formula
C10H13N5O12P3Na2
C9H14N3O13P3Na2
C9H13N3O13P3Na2
C10H16N2O14P3Na2 |
Molecular Weight
535.15
511.12
573.13
504.15 |
Each ultra pure 2'-deoxynucleotide 5'-triphosphate is diluted in a stabilized solution (pH 7.0).
Quality Control
- ≥ 99% purity as estimated by HPLC.
- Preparation is free of DNase and RNase contamination as determined by incubation of the 4 dNTPs with radioactive substrates.
- The concentration has been verified by an optical density spectrophotometer.
- This lot of dNTPs has been tested with Sequenase, Taq DNA polymerase, pfu DNA polymerase and T7 DNA polymerase.
Also
dCTP is sodium salts, solution, 100 mM, pH8.3, > 99% dCTP (HPLC), < 0.9% dCDP
dATP is sodium salts, solution, 100 mM, pH8.3, > 99% dATP (HPLC), < 0.9% dADP
dGTP is sodium salts, solution, 100 mM, pH8.3, > 99% dGTP (HPLC), < 0.9% dGDP
dTTP is sodium salts, solution, 100 mM, pH8.3, > 99% dTTP (HPLC), < 0.9% dTDP
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