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RNase A Let's See What We Have Here

Description

RNase A is a ready-to-use solution chromatographically purified. It is a pyrimidine specific endonuclease acting on single-stranded RNA. One unit is defined as the amount of enzyme that will catalyze the hydrolysis of RNA to yield a first-order velocity constant equal to 1.0 at 25C, pH 5.0

Applications

  • Aid DNA extraction by specifically degrading RNA in nucleic acid mix
  • RNase Protection assay
  • RNase A can hydrolyze RNA contamination in protein samples

Specifications

  • Format : Liquid Purity: >95% by SDS-PAGE
  • Activity : >100 U/mg Proteases (not detected)
  • Dnase I : (not detected)
Protocol: HIGH Purity RNase A, Bovine Pancreas

Components: 1.5 ml Ribonuclease A (RNase)

RNase A Preparation: RNase A was prepared by solubililzing 150 mg of lypholized RNase A from Bovine pancreas in 0.01M NaOAC (pH 5.2). The RNase preparation was heated to 100°C for and incubated here for a duration of 15min. The RNase A solution was cooled to room temperature, and the pH was adjusted 7.0 via the addition 0f 1 M Tris (not pH adjusted).

To ensure maximum performance of RNase A we recommend measuring the pH prior to use and adjusting to pH of 7.0 if required.

Purity: RNase A has been purified using a proprietary chromotographic method, which eliminates all Dnase enzyme which may be present in the crude enzyme sample. As a result of this process Rnase A (Cat# RNASE100-01) is fully certified to contain no Dnase contamination.

Units: 70 Kunitz units/mg protein

 Quantity   Cat. No.   Product Name   Size   Unit   Price Per Unit   Price 
 RNASE100-01  RNase A  1.50   100 mg/ml   $36.67   $55.00 

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