The E.Z.N.A.^® Mollusc RNA Kit is designed for efficient recovery of total RNA greater than 200 nucleotides from molluscs, arthropods, roundworms, flatworms, and other invertebrate tissue samples rich in mucopolysaccharides.

Principle

The E.Z.N.A^® Mollusc RNA Kits combines the reversible nucleic acid-binding properties of HiBind^® matrix with the speed and versatility of spin column technology. HiBind™ matrix avidly, but reversibly, binds RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with de-ionized water or low salt buffer. The procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective RNA binding of HiBind^® matrix.

Procedure

Samples are homogenized and lysed in a high salt buffer containing CTAB and extracted with chloroform to remove mucopolysaccharides and to denature proteins. Following a rapid alcohol precipitation step, binding conditions are adjusted and RNA further purified using HiBind^® RNA spin columns. In this way, salts, proteins and other contaminants are removed to yield high quality total RNA suitable for downstream applications.

Applications

RNA purified using the E.Z.N.A.^® Mollusc RNA Kit can be used in applications such as :

• Reverse Transcription
• Hybridization Techniques
• Poly A⁺ RNA selection