E-Z 96^® Tissue RNA Isolation Kit is designed for the isolation of total RNA from animal tissues such as skeletal muscle, heart and aorta tissue. The kit allows for the simultaneous processing of up to 192 samples in less than 60 min The procedure completely removes contaminants and enzyme inhibitors making RNA isolation fast, convenient, and reliable.

Principle

The E-Z^® Tissue RNA Kits use the reversible binding properties of HiBind^® matrix, a new silica-based, time-saving spin technology material. Combined with the speed of 96-well spin or vacuum manifold technology, up to 192 samples can be processed at same time. It combines the reversible nucleic acid-binding properties of the HiBind™ matrix with the speed and versatility of the E-Z 96™ RNA plate to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from tissue lysates.

Procedure

The samples are lysed first under highly denaturing buffer conditions so that RNases will be inactivated, and the intact Tissue RNA is protected from degradation. After adjusting the binding conditions, the samples are loaded to the E-Z 96^® RNA Plate. With a brief centrifugation or vacuum, the samples pass through the plate and the RNA binds to the Hibind^® matrix. After two wash steps, purified Tissue RNA will be eluted with RNase-free water.

Applications

RNA purified using the E-Z 96^® Tissue RNA Isolation Kit can be used in applications such as :

• RT-PCR