The E.Z.N.A.^® Total RNA Maxiprep Kit uses HiBind^® matrix spin-column technology to isolate up to 5 mg total cellular RNA from a variety of sources including up to 1 g tissue and as many as 5 x 108 cultured cells.

Principle

By using the HiBind^® Maxi spin-column technology, this E.Z.N.Asup>® Total RNA Maxiprep Kit greatly reduces the processing time when compared to traditional phenol methods. The E.Z.N.A.^® Total RNA Maxiprep Kit uses the reversible binding properties of the HiBind^® matrix, a new silica-based material. A specially formulated high salt buffer system allows more than 1mg of RNA molecules greater than 200 bases to bind to the matrix.

Procedure

Samples are lysed and homogenized in denaturing conditions to ensure degradation of proteins and inactivation of endogenous RNases. Binding conditions are then optimized to favor RNA isolation before the resulting cell lysates are passed through the HiBind^® RNA Maxi spin-columns. Subsequent wash steps remove cellular debris and trace salt contaminants to allow elution of pure RNA in DEPC-treated water.

Applications

RNA purified using the E.Z.N.A.^® Total RNA Maxiprep Kit can be used in applications such as :

• Reverse Transcription
• Northern Analysis
• RNase protection Assays
• Differential display