The E.Z.N.A.^® Poly-Gel DNA Extraction Kit combines the simplicity of PAGE with the power of HiBind^® technology to allow rapid and consistent recovery of ssDNA or dsDNA from acrylamide gels. Following PAGE, oligonucleotides are first visualized by UV shadowing or ethidium bromide staining and then eluted for 1-4 hours using EDTA-containing buffer. A specially formulated buffer is then added and the whole mixture is passed through a HiBind^® DNA spin column. The bound DNA is washed to remove salts, buffers, and any protein contaminants, and then eluted with sterile deionized water or low salt buffer. The spin column format allows parallel processing of multiple samples.

Procedure

Applications

E.Z.N.A.^® Poly-Gel DNA Extraction Kit can be used for the following applications :

• PCR
• Ligation Reactions
• Hybridization Techniques