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HiYield Gel/PCR DNA Extraction

Up to 90% recovery from gel slice

Sample size : 200 mg of agarose gel, 100 µl of PCR solution
Gel Recovery : 70-80%
PCR Recovery : 80-90%
Preparation time: 20 minutes
Elution volume : 15~50 µl

Protocol: HiYield Gel/PCR Extraction Kit

Description

The HiYield™ Gel/PCR DNA Extraction Kit is designed to recover or concentrate DNA fragments (50bp-10Kb) from agarose gels, PCR or other enzymatic reactions. The unique dual purpose application and high yield DNA/mini column make this kit an exceptional value. The method uses a chaotropic salt, guanidine thiocyanante to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of the spin column. Following washing off of contaminants, the purified DNA fragments are eluted by addition of low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation.

Applications

Unique dual purpose application
DNA Sequencing
DNA library Screening and Analysis
Restriction Digestion
Preparation of PCR probes for microarrays
Ligation and Transformation

* Reference: Vogelstein, B., and Gillespie, D. (1979) Proc. Natl. Acad. Sci. USA 76, 615.

Cat. No. YDF100
100 mini Prep / kit
DF Buffer: 80 ml
Wash Buffer (concentrated): 20 ml
Elution Buffer: 6 ml
DF Columns: 100 pcs
Collection Tubes: 100 pcs

Cat. No. Cat. No. YDF300
300 mini Prep / kit
DF Buffer: 240 ml
Wash Buffer (concentrated): 50 ml
Elution Buffer: 30 ml
DF Columns: 300 pcs
Collection Tubes: 300 pcs

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