Excess unincorporated dye terminators can cause high background fluorescence during automated sequencing. The E.Z.N.A^® Mag-Bind 96^® Dye terminator Removal Kit is designed to clean-up dye terminator reactions prior to automated sequencing analysis. The system is ideally suited for fully automated purification of dye terminator clean-up. Sequence quality is similar to or better than standard manual methods and offers the distinct advantage of a hands-off protocol.

Principle

Magnetic particles offer greater flexibility than centrifugation and vacuum based formats for nucleic acid purification. Among these benefits are scalability, easier handling and in-solution kinetics. The purification process results in highly purified sequencing products.

Procedure

Transfer the samples into the provided 96 well micro plate. Add 2 volumes of MagBead^® Particle /Ethanol and mix thoroughly by pipetting. Incubate 10 minutes at room temperature. Place the micro plate on a magnetic separation device and allow the solution to clear (approximately 1minute). Aspirate entire solution and remove the magnet. Wash the MagBead™ Particle pellet with MPG Wash Buffer, aspirate supernatant, air dry for 10 minutes and elute with distilled water.

DNA purified using E.Z.N.A^® Mag-Bind 96^® Dye terminator Removal Kit can be used for the following applications :

• Automated Sequencing Analysis