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Micro-Spin Gel Extraction Kit
For purification of DNA fragment from gel with elution volume

  • Elution with as little as 10µl deionized water or Tris Buffer
  • 10µg DNA binding capacity
  • Purify DNA from agarose gel in less than 20 minutes
  • >80% recovery of fragments
  • Gel extraction from regular or low-melt agarose gel
  • New Binding Buffer with pH indicator
Protocol: E.Z.N.A.® Micro Spin Gel Extraction Kit


Introduction

The MicroSpin® DNA Clean-up system is a convenient system for fast and reliable purification of DNA from agarose gels with a relatively small elution volume of 10- 15µl.

Principle

This method uses HiBind® technology to recover DNA bands 60 bp-40 kb DNA free of agarose, oligonucleotides, nucleotides, and enzymes in yields exceeding 80%. Key to the system is the new HiBind® matrix that specifically, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.

Procedure

Binding conditions are adjusted by addition of a specially formulated buffer, and the sample is applied to a HiBind® DNA spin-column. Following a rapid wash step, DNA is eluted with deionized water (or low salt buffer) and ready for other applications.

Applications

E.Z.N.A.® Micro-Spin Gel Extraction Kit can be used for the following applications :

  • T-A Ligations
  • Restriction digestion
  • Labeling Reactions
  • PCR Sequencing

 Quantity   Cat. No.   Product Name   Size   Unit   Price Per Unit   Price 
 D6294-01  E.Z.N.A.® Micro Spin Gel Extraction Kit  50.00   Prep   $1.32   $66.00 
 D6294-02  E.Z.N.A.® Micro Spin Gel Extraction Kit  200.00   Prep   $1.18   $236.00 
Competitor  28604  Qiagen MinElute Gel Extraction Kit  50.00   Prep   $2.30   $115.00 

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DNA and RNA Kits-> (218)
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