The E.Z.N.A.^® family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. Key to the system is the new HiBind^® matrix that specifically, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.

Description

The DNA Probe Purification Kit is a convenient system for fast and reliable purification of DNA from random-primed or nick-translation labeling reactions.

Procedure

Salts, enzymes and free label (radioisotopic or not) are effectively removed to yield pure DNA probes. Binding conditions are adjusted by addition of a specially formulated buffer, and the sample is applied to a HiBind^® DNA spin-column. Following two rapid wash steps, DNA is eluted with deionized water (or low salt buffer) and ready for other applications. No organic extractions or alcohol precipitations means safe and rapid processing of multiple samples in parallel.

1. Determine volume of reaction. Add 5 volumes of Buffer DP. Include rRNA if DN A is labeled with radioisotopic nucleotide(s).






2. Apply solution to HiBind™ DNA column assembled in 2ml collection tube.






3. Centrifuge at maximum speed 1 min at room temperature. Discard liquid.






4. Wash column twice with 750 ul Wash Buffer diluted with ethanol. Optional: repeat wash step.






5. Centrifuge empty column 1 min at max speed to dry.






6. Place column into clean 1.5 ml tube and elute DNA with 30-50 ul sterile water or TE buffer. Centrifuge 1 min.