E-Z 96® Blood DNA Kit allows rapid and reliable isolation of high-quality genomic DNA from a wide variety of sample sources including fresh, frozen, or anti-coagulated whole blood; serum; plasma; bone marrow; other body fluids; lymphocytes and cultured cells. By adapting the HiBind® technology with the 96 well plate, the E-Z 96® Blood DNA Kit provides a high-throughput method to purify genomic DNA from up to 200 µl healthy whole blood in a 96-well format. The streamlined E.Z.N.A.® procedure and convenient 96- well format allows processing of 96 samples in less than 2 hours. The E-Z 96® system is designed and optimized for use with vacuum manifolds and is compatible with most pipetting machines.

Principle

The E-Z 96® DNA kit incorporates the reversible nucleic acid-binding properties of HiBind® matrix in a high throughput 96-well format to eliminate proteins, nucleases and other enzyme inhibitors or contaminators from blood or body fluids. Up to 96 samples can be simultaneously processed in a single E-Z 96® DNA plate. The newly designed E-Z 96® DNA plate has a binding capacity of 100 µg DNA per well. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.

Procedure

Blood or other body fluid samples are added to a specially formulated buffer containing detergent and mixed with proteinase. Binding conditions are then adjusted and the sample is applied to the E-Z 96® DNA plate. Two rapid wash steps remove trace contaminants and enzyme inhibitors and pure DNA is eluted in water or low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Applications

DNA purified using the E-Z 96® DNA kit is suitable for the following applications :

• PCR
• Restriction Digestion
• Hybridization Techniques