The E-Z 96^® Mag-Bind^® Plasmid Isolation Core Kit is a magnetic bead-based, plasmid purification system that uses a single protocol to purify a variety of high and low copy number plasmids. This flexible system is easily automated without sacrificing product quality. The single protocol, ease of automation, and high quality results make Mag-Bind^® Plasmid Isolation Kit an ideal solution for all your plasmid purification needs.

Principle

The E-Z 96^® Mag-Bind^® Plasmid Isolation Kit combines the power of MagBead^® technology with the time-tested consistency of alkaline- SDS lysis of bacterial cells to deliver high quality plasmid DNA. Mag-Bind^® Particle avidly, but reversibly, binds DNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with de-ionized water or low salt buffer. By using 96-well format, up to 96 samples can be simultaneously processed in less than 60 minutes. The new E-Z 96^® Lysate Clearance Plate obviates time-consuming centrifugation for clearing of the bacterial alkaline lysates. It also has an average DNA recovery rate 10 to 30% higher than the manual centrifuge method.

Procedure

Bacterial cells grown in 96 deep well plate are pelleted by centrifugation and re-suspended in Solution I/RNase A. Add Solution II to obtain a cleared lysate and neutralization buffer and mix well until a flocculent white precipitate forms. Cell lysates are then cleared with E-Z 96^® Lysate Clearance Plate. Transfer the cleared lysate to the 96-well Binding plate and add Mag-Bind^® Particles Solution E and MGC Binding Buffer. After mixing and incubation, Plasmid DNA are selectively bound to MagBead Particles. Wash the Mag-Bind^® particles twice with SPM wash buffer, air-dry them and elute the purified DNA with water or TE Buffer.

Applications

E-Z 96^® Mag-Bind^® Plasmid Isolation Kit provides high quality, reproducible plasmid DNA for use in the following applications :

• Automated Fluorescent DNA sequencing
• Restriction Digestion