Update March 2006
Filter columns and collection tubes have been redesigned for smother polish to reduce nucleic acid adherence to the plastic and better sample handling. Extra membrane layers have been added for improved retention of nucleic acids.
Description
HiYield Plasmid Mini Kit is designed for rapid isolation of plasmid or cosmid DNA from 1-4 ml of bacterial cultures. The high yield/mini column ensures enough DNA for many subsequent applications. The process follows the modified alkaline lysis method. RNase treatment is used to get cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of a chaotropic salt, the plasmid DNA in the lysate binds to glass fiber matrix in the spin column. Contaminants are washed with an ethanol wash buffer. In the final step the purified plasmid DNA is eluted by low salt elution buffer or distilled water. For large scale plasmid purification we recommend ion exchange based Fast Ion Plasmid Mini/Maxi Kits.
Applications
DNA sequencing, DNA library screening and analysis, Restriction Digestion, Ligation and Transformation.
* Reference: Birnboim, H. C., and Doly, J. (1979) Nucleic Acids Res. 7, 1513.Vogelstein, B., and Gillespie, D. (1979) Proc. Natl. Acad. Sci. USA 76, 615.
Quality Control
The quality of HiYield Plasmid Mini Kits are tested on a lot-to-lot basis. The Kits are tested by isolation of plasmid DNA from 4 ml culture of E. coli DH5a transformed with the plasmid pBluescript (A600 >2 units/ml)
HiYield Plasmid Mini Kit : Yield, Purity and Stability Test
Each Sample Tested 3X
| Real Genomics |
DH5α/TA |
BL21/pET20b |
| Volume (µl) |
139.4 |
115.8 |
123.5 |
19.7 |
18.7 |
18 |
| DNA conc. (ng/µl) |
139.4 |
115.8 |
123.5 |
19.7 |
18.7 |
18 |
| Total DNA (µg) |
5.99 |
5.01 |
5.43 |
0.88 |
0.83 |
0.78 |
| A260>/A280 |
1.92 |
1.91 |
1.91 |
1.78 |
2.03 |
1.82 |
| Brand Leader Q |
DH5α/TA |
BL21/pET20b |
| Volume (µl) |
44.5 |
44.5 |
45 |
46 |
44 |
45 |
| DNA conc. (ng/µl) |
112.2 |
10.8 |
105.7 |
20.6 |
24.8 |
19.9 |
| Total DNA (µg) |
4.99 |
4.49 |
4.76 |
0.95 |
1.09 |
0.9 |
| A260>/A280 |
1.91 |
1.9 |
1.89 |
1.78 |
1.72 |
1.84 |
Cat. No. YPD100
100 mini Prep / kit
PD1 Buffer: 25 ml
PD2 Buffer: 25 ml
PD3 Buffer: 45 ml
W1 Buffer: 45 ml
Wash Buffer (concentrated): 12.5 ml
Elution Buffer: 6 ml
RNase A (50 µg/µl): 50 µl
PD Columns: 100 pcs
2 ml Collection Tubes: 100 pcs |
Cat. No. YPD300
300 mini Prep / kit
PD1 Buffer: 65 ml
PD2 Buffer: 75 ml
PD3 Buffer: 100 ml
W1 Buffer: 130 ml
Wash Buffer (concentrated): 50 ml
Elution Buffer: 20 ml
RNase A (50 µg/µl): 130 µl
PD Columns: 300 pcs
2 ml Collection Tubes: 300 pcs |
