The E.Z.N.A.^® Micro-Spin Total RNA Kits combine the reversible binding properties of HiBind^® matrix, a new silica-based material with the speed of mini-column spin technology. A specifically formulated high salt buffer system allows RNA molecules greater than 200 bases to bind to the matrix. Cells or tissues are first lysed under denaturing conditions that inactivate RNases. Ethanol, samples are then applied to the HiBind^® Micro-spin columns to which total RNA binds , while cellular debris and other contaminants are effectively washed away. High quality RNA is finally eluted in DEPC treated sterile water.

Description

The E.Z.N.A.^® Micro-Spin Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amount of cultured eukaryotic c ells, tissues such as laser dissected samples (LDS) or fine needle aspirates (FNA). Normally, up to 5 x 10⁵ eukaryotic cells or 5 mg tissue (amounts depend on the tissue) can be used in a single experiment. The kit allows single or multiple, simultaneous processing of samples in less than 30 min. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl, are eliminated.

Applications

RNA purified using the E.Z.N.A.^® Micro-Spin Total RNA method is ready for the following applications :

• RT-PCR*
• Northern Blotting
• poly A+ RNA (mRNA) purification
• nuclease protection
• in vitro translation