The MicroSpin^® Cycle-Pure Kit is a convenient system for fast and reliable purification of DNA from PCR reactions with a relatively small elution volume of 10- 15µl.

Principle

This method uses HiBind^® technology to recover DNA bands 60 bp-40 kb DNA free of agarose, oligonucleotides, nucleotides, and enzymes in yields exceeding 80%. Key to the system is the new HiBind^® matrix that specifically, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.

Procedure

Binding conditions are adjusted by addition of a specially formulated buffer, and the sample is applied to a HiBind^® DNA spin-column. Following a rapid wash step, DNA is eluted with deionized water (or low salt buffer) and ready for other applications.

Applications

E.Z.N.A.^® Micro-Spin Cycle-Pure Kit can be used for the following applications :

• T-A Ligations
• Restriction digestion
• Labeling Reactions
• PCR Sequencing