The E.Z.N.A.^® Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects and invertebrates. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material.

Principle

The E.Z.N.A.^® Insect DNA Kit relies on the well established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of HiBind^® Matrix. The use of mini-columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be simultaneously processed. For more information on columns, click Flexible Multi-Format Columns.

Procedure

Samples are homogenized and lysed in a high salt buffer containing CTAB and extracted with chloroform to remove mucopolysaccharides. Following a rapid alcohol precipitation step, binding conditions are adjusted and DNA further purified using HiBind DNA spin columns. In this way salts, proteins and other contaminants are removed to yield high quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.

Applications

DNA purified using the E.Z.N.A.^® Insect DNA Kit is suitable for the following applications :

• PCR
• Restriction Digestion
• Hybridization Techniques