E-Z 96^® MagBeads^® Fungal DNA Kits provides rapid and reliable isolation of high-quality genomic DNA from a wide variety of fungal species and tissues. Up to ninety-six 50 mg samples of wet tissue (or 15 mg dry tissue) per micro plate can be processed in less than 1 hour. The system combines Omega Bio-Tek’s EaZy Nucleic Acid^® buffer chemistry with the convenience of MagBeads^® particles to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions, thus reducing plastic waste and hands on time allowing multiple samples to be processed in parallel.

Procedure

Dry or fresh fungal sample is disrupted and then lysed in a specially formulated buffer containing detergent. Proteins, polysaccharides, and cellular debris are subsequently precipitated. The lysate is transferred to a 96-well micro plate, binding conditions are adjusted, and genomic DNA binds to the MagBeads^® particles. One or two rapid wash steps remove trace contaminants such as residual polysaccharides. Finally pure DNA is eluted in water or low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.