E.Z.N.A.^® Fungal DNA Mini Kits allow rapid and reliable isolation of high-quality total cellular DNA from a wide variety of fungal species and tissues. Up to 200 mg of wet tissue (or 50 mg dry tissue) can be processed in less than 1 hour.

Principle

The E.Z.N.A.^® Fungal DNA Kit combines the reversible nucleic acid-binding properties of HiBind^® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. The use of mini-columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be simultaneously processed. For more information on columns, click Flexible Multi-Format Columns.

Procedure

Dry or fresh tissue is disrupted and then lysed in specially formulated buffers containing detergent. Proteins, polysaccharides, and cellular debris are subsequently precipitated. Contaminants are further removed by isopropanol precipitation of the DNA. Binding conditions are then adjusted and the sample is applied to a HiBind^® DNA spin-column. Two rapid wash steps remove trace contaminants such as residual polysaccharides, and pure DNA is eluted in water or low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Applications

DNA purified using the E.Z.N.A.^® Fungal DNA Kit is suitable for the following applications :

• PCR
• Restriction Digestion
• Hybridization Techniques