The E.Z.N.A^® Mag-Bind^® Forensic DNA System is designed for the purification of DNA from forensic samples such as dried blood, and buccal swabs. This system is ideal to prepare clean samples for short tandem repeat (STR) analysis’ easily and efficiently because it provides small but fixed yields from any type of samples.

Principle

The E.Z.N.A^® Mag-Bind^® Forensic DNA Kit uses paramagnetic particles, considered a "mobile solid phase". The system combines the reversible nucleic acid-binding properties of Mag-Bind^® particles with the time-proven efficiency of SP plant lysis buffer system to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Unlike column-based systems, the binding of nucleic acids to magnetic particles occurs in solution, resulting in increased binding kinetics and binding efficiency. Particles are also completely re-suspended during the wash steps of the purification protocol, thus enhancing contact with the wash buffer and removal of contaminants and increasing nucleic acid purity.

Procedure

The forensic sample tissue is disrupted and then lysed in a specially formulated buffer. Proteins, polysaccharides, and cellular debris are subsequently precipitated. The lysate is transferred to a new tube and binding conditions are adjusted so that genomic DNA will selectively bind to the MagBead^®. Two rapid wash steps remove trace contaminants such as residual polysaccharides, and pure DNA is eluted in Elution Buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Applications

DNA purified using the E.Z.N.A.^® Mag-Bind^® Forensic DNA Kit is suitable for the following applications :

• PCR
• Restriction Digestion
• Hybridization Techniques
• RAPD analysis