By adapting the HiBind^® technology with 96 well plates, the E-Z 96^® Plant DNA Kit provides a high-throughput method to purify cellular DNA from 10 mg plant tissue in a 96-well format. The streamlined E.Z.N.A.^® procedure and convenient 96- well format allow processing of 96 samples in less than 2 hours. The E-Z 96^® system is designed and optimized for use with vacuum manifolds and is compatible with most pipetting machines.

Principle

The E-Z 96^® Plant DNA Kit combines the reversible nucleic acid-binding properties of the HiBind™ matrix with the speed and versatility of the E-Z 96™ DNA plate to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates.

Procedure

Dry or fresh plant tissue is disrupted and then lysed in a specially formulated buffer. Proteins, polysaccharides, and cellular debris are subsequently precipitated. Contaminants are further removed by isopropanol precipitation of DNA. Binding conditions are then adjusted and the sample is applied to an E-Z 96™ DNA plate. Two rapid wash steps remove trace contaminants such as residual polysaccharides, and pure DNA is eluted in water or low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Applications

DNA purified using the E-Z 96^® Plant DNA Kit is suitable for the following applications :

• PCR
• Restriction Digestion
• Hybridization Techniques