E.Z.N.A.™ Plant Maxiprep Kits allow rapid and reliable isolation of high-quality total cellular DNA from a wide variety of plant species and tissues. Up to 2 g of wet tissue (or 500 mg dry tissue) can be processed in less than 1 hour.

Principle

The system combines the reversible nucleic acid-binding properties of HiBind™ matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.

Procedure

Dry or fresh plant tissue is disrupted and then lysed in a specially formulated buffer containing detergent. Proteins, polysaccharides, and cellular debris are subsequently precipitated. Contaminants are further removed by isopropanol precipitation of DNA. Binding conditions are then adjusted and the sample is applied to a HiBind™ DNA spin-column. Two rapid wash steps remove trace contaminants such as residual polysaccharides and pure DNA is eluted in water or low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Applications

DNA purified using the E.Z.N.A.^® Plant DNA Maxiprep Kit is suitable for the following applications :

• Restriction Digestion
• Hybridization Techniques
• PCR

Electrophoresis analysis (1% agarose gel in 0.5 x TBE)
of isolated DNA from leaves of Sweet potato (Lane 1),
Cherry (lane 2), Maize (lane 3). MW: Lambda DNA
digested with Hind III.