The E.Z.N.A.^® HP Tissue DNA Midi Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from up to 500 mg of tissue samples. The special designed buffer system ensure the optimal lysis of tissue rich in fat, polysaccharides and fibers such as brain, adipose, muscles. This also can isolate DNA from molluscs, insects, arthropods, roundworms, flatworms, and other invertebrate tissue samples rich in mucopolysaccharides. The procedure relies on the well established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-Tek’s HiBind^® matrix.

Procedure

Samples are homogenized and lysed in a high salt buffer containing CTAB and digested with proteinase. After addition of chloroform, the homogenate is separate into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted and BL buffer and are added to provide appropriate binding conditions. The sample is then loaded into the HiBind^® DNA Spin Column, where the genomic DNA binds to the membrane and salt and other contaminants are efficiently washed way. High quality genomic DNA is then eluted with elution buffer or water.

Applications

Purified DNA is suitable for most downstream applications such as :

• PCR
• Restriction Digestion
• Hybridization Techniques