The E.Z.N.A.® Blood DNA Maxiprep Kits are designed for isolation of genomic DNA from up to 30 ml fresh, frozen and anti coagulated whole blood treated with any common anticoagulant such as heparin, EDTA, or acid-citrate -dextrose. 30 ml blood typically yields 400μg-1.2mg of genomic DNA. OB Protease is included in this kit.

Principle

The E.Z.N.A.® Blood DNA Maxiprep Kits use the reversible binding properties of HiBind® matrix, a new silica-based material. This is combined with the speed o f midi-column spin technology. HiBind™ matrix avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with de-ionized water or low salt buffer. The use of maxi-columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be simultaneously processed. For more information on columns, click Flexible Multi-Format Columns.

Procedure

Red blood cells are selectively lysed and white cells collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate DNases, genomic DNA is purified on the HiBind® Maxi spin column. A specifically formulated high salt buffer system allows DNA molecules greater than 200 bases to bind to the matrix. Cellular debris and other contaminants (such as hemoglobin) are effectively washed away and high quality DNA is finally eluted in DNA Elution Buffer.

Applications

DNA purified using the E.Z.N.A.® Maxiprep Blood DNA Kit is suitable for the following applications :

• PCR
• Restriction Digestion
• Southern blotting