Quality, affordable solutions for your life sciences needs.
United Bioinformatica Inc Bioinformatics Software Products Bioinformatics Services Site Map of UBI Contact UBI Search in United Bioinformatica Inc
United Bioinformatica Inc.
  Top » Catalog » Polymerases » Pfu DNA Polymerase My Account  |  Cart Contents  |  Checkout   
Pfu DNA Polymerase

Description

Recombinant Pfu DNA polymerase is produced in E. coli utilizing a gene isolated from the hyperthermophilic marine archaebacterium Pyrococcus furiosus. This multifunctional thermostable enzyme possesses both 5’- to 3’- DNA polymerase and 3’- to 5’- exonuclease activity, resulting in a 12-fold increase in fidelity during DNA synthesis compared to conventional Taq DNA polymerase. UBI’s Recombinant Pfu DNA Polymerase has an optimal extension temperature between 72°C and 78°C. The enzyme remains more than 95% active following a one hour incubation at 95°C.
Protocol: Recombinant Pfu DNA Polymerase


10 x Reaction Buffer

  • 200 mM TrisHCl ( pH 8.8)

  • 100 mM KCl

  • 100 mM (NH4)2 SO4

  • 20 mM Mg SO4

  • 1% Triton X-100

  • 1 mg / ml nuclease-free Bovine Serum Albumin ( BSA )


Unit Activity

One unit of activity is defined as the amount of Recombinant Pfu DNA polymerase required to incorporate 10nM of [H]TTP into an acid-insoluble form after 30 minutes at 72°C.


Contaminants

Recombinant Pfu DNA polymerase has been assyed for the presence of detectable non-specific nuclease activity and DNA contamination and has been deemed nuclease and DNA free.


Shipping Conditions

Recombinant Pfu DNA polymerase is shipped frozen in the presence of dry ice, but is stable and maintains full activity if stored at room temperature.


Storage Conditions

Recombinant Pfu DNA polymerase is stored in 50mM Tris-HCL (pH8.2), 1mM dithiothreitol (DTT), 0.1mM EDTA, 0.05% CHAPS and 50% (v/v) glycerol. Recombinant Pfu DNA polymerase although stable at room temperature should be stored at -20°C on receipt for maximal life.

 Quantity   Cat. No.   Product Name   Size   Unit   Price Per Unit   Price 
 PFU-01  Recombinant Pfu DNA Polymerase  500.00   unit   $0.40   $199.00 

To order, enter the Quantity for the product(s) desired and click 'Add to Cart'

Checkout Reviews

United Bioinformatica Inc
Search in United Bioinformatica Inc

Site Map of UBI

Contact UBI
About Us
People
Partners
Customers
Support
Free Applications
Charity

Categories
Cloning Systems-> (6)
Competent Cells-> (17)
Deoxynucleotides (6)
DNA and RNA Kits-> (219)
DNA Ladders-> (7)
Enzymes-> (7)
Polymerases-> (3)
  HP Taq DNA Polymerase (2)
  Pfu DNA Polymerase (1)
Special Order-> (69)
Manufacturers
Quick Find
 
Use keywords to find the product you are looking for.
Information
Shipping & Returns
Privacy Notice
Conditions of Use
Contact Us
Send To A Friend more
Refer a new customer to UBI by clicking on the button below and when your friend orders products over $300 (excluding taxes and shipping) the first time, you both receive a $20 gift card to Tim Horton's! Also, every time you refer a friend, you are automatically entered in a contest to receive a $500 pre paid VISA. Enter as many times as you like! Contest ends on June 15, 2008.
Send to a Friend
Specials more

1kb Ready to Use DNA Ladder
$65.00

Send mail to webmaster with questions or comments about this web site.
Copyright 2001-2005 United Bioinformatica Inc.
Powered by FoundPages.