Description

Glass plating beads (4 mm) provide and efficient and easy method to achieve optimal spreading on agar plates. The rolling action of the glass beads gently spreads the E. coli solution (or any bacterial/fungal solution) to reach areas of the plate that are inaccessible to spreaders. Multiple plates containing beads and E. coli solution may be stacked and shaken simultaneously to increase spreading through out and reduce cell stress. The beads may be recovered by washing with 70% ethanol sterilized by autoclaving and oven dried.


Applications

• Increase transformation efficiency


• Rapid simultaneous plating


• Economical: 75-100 plates/bottle


• Reusable


• Sterile

For regular plating of RBC HIT™ cells 10-20 beads may be added immediately after pipetting 50-100 ml of transformed cells onto each plate. Move the plate back and forth to roll the beads on the surface and change the direction of shaking a few times to ensure even spreading. When the surface of the agar visibly begins to dry on the plate (20-40 seconds) pour off the beads and the plates are ready for incubation.